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The University of Florida is one of sixty-two elected member institutions of the Association of American Universities (AAU), the association of preeminent North American research universities, and the only AAU member university in Florida.

Andrew Sledd, president of the pre-existing University of Florida at Lake City, was selected to be the first president of the new University of the State of Florida.

After the Florida state legislature's creation of performance standards in 2013, the Florida Board of Governors designated the University of Florida as one of the two "preeminent universities" among the twelve universities of the State University System of Florida.

The University of Florida is home to sixteen academic colleges and more than 150 research centers and institutes.

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The Buckman Act also consolidated the colleges and schools into three institutions segregated by race and gender—the University of the State of Florida for white men, the Florida Female College for white women, and the State Normal School for Colored Students for African-American men and women.

On July 6, 1905, the Board of Control selected Gainesville for the new university campus.

In their 108-year history, the university's varsity sports teams have won thirty-five national team championships, thirty of which are NCAA titles, and Gator athletes have won 275 individual national championships.

The University of Florida traces its origins to 1853, when the East Florida Seminary, the oldest of the University of Florida's four predecessor institutions, was founded in Ocala, Florida.

The Buckman Act organized the State University System of Florida and created the Florida Board of Control to govern the system.